Publications

2025

Chen YC^#, Sun JY^#, Shi KL^#, Cui YL*, Wu B*, et al. Glycolysis-compatible urethanases for polyurethane recycling. Science 2025.

Sun JY, Zhu T, Cui YL*, Bian W*, et al. Structure-based Self-Supervised Learning Enables Ultrafast Protein Stability Prediction Upon Mutation. The Innovation 2025, 6(1). [link]

Tang F, Sun J, Wu B*, et al. Reliable Prediction of Protein-Protein Binding Affinity Changes Upon Mutations with Pythia-PPI. Natl. Sci. Rev. 2025, 12(6): nwaf231. [link]

Chen Z, Xu J, Wu B*, et al. Small RNA Toxin-Assisted Evolution of GC-Preferred ErCas12a for Enhanced Genome Targeting Range. Adv. Sci. 2025, 12(29): e17105. [link]

2024

Tan Y, Tang X, Wu B*,Shao F*,Chen YH*, et al. The β-D-Manno-Heptoses Are Immune Agonists Across Kingdoms. Science 2024, 385(6709): 678-684. [link]

Cui YL, Chen YC, Geng WC*, Wu B*, et al. Computational Redesign of a Hydrolase for Nearly Complete PET Depolymerization at Industrially Relevant High-Solids Loading. Nat. Commun. 2024, 15(1). [link]

Zhu T, Sun JY, Wu B*, et al. Computational Enzyme Redesign Enhances Tolerance to Denaturants for Peptide C-Terminal Amidation. JACS Au 2024, 4(2): 788-797. [link]

Zhu T, Zhang X, Wu B*, et al. Efficient Production of Peptidylglycine α-Hydroxylating Monooxygenase in Yeast for Protein C-terminal Functionalization. Int. J. Biol. Macromol. 2024, 263: 130443. [link]

Sun JY, Song W, Wu B*, et al. GRAPE-WEB: an Automated Computational Redesign Web Server for Improving Protein Thermostability. mLife 2024, 3(4): 527-531. [link]

Chen J, Cai Y, Wu B*, Huang J, Xu H, et al. Unveiling the Repressive Mechanism of a PPS-like Regulator (PspR) in Polyhydroxyalkanoates Biosynthesis Network. Appl. Microbiol. Biotechnol. 2024, 108(1): 1-10. [link]

Deng S, Zhang JS, Wu B* et al. Recent Advances in Chemical Protein Synthesis: Method Developments and Biological Applications. Sci. China Chem. 2024: 1-37. [link]

Zhu T, Wu B*. Repurposing Amide Bond-Forming Enzymes for Non-native Protein Modification. ACS Catal. 2024, 14(21): 15811-15826. [link]

Li X, Gu W, Wu B*, et al. Structure Motif Guided Mining of MHET Hydrolase and Development of a Two-Enzyme Cascade for Plastics Depolymerization at Mild Temperature. Chin. J. Biotechnol. 2024, 40(3): 773-785. [link]

Zhang H, Zhu T, Wu B*, et al. Two-step Computational Redesign of Bacillus subtilis Cellulase and β-Glucanase for Enhanced Thermostability and Activity. Int. J. Biol. Macromol. 2024, 242: 138274. [link]

2023

Ren SY^#, Geng WC^#, Cui XX, Wu B*, Zheng Z*, et al. A Label-Free and Continuous Fluorescence Detection for L-DOPA Decarboxylase Activity Based on Supramolecular Tandem Assay. ChemistrySelect 2023, 8(2), e202203646. [link]

Chen ML^#, Guo ZY^#, Sun JY^#, Tang W, Wu B*, Chen YH*, et al. Insights into the biosynthesis of septacidin l-heptosamine moiety unveils a VOC family sugar epimerase. Acta Pharm. Sin. B 2023, 13(2), 765-774. [link]

2022

Cui XX^#, Geng WC^#, Jiang HF, Wu B*, et al. Photobiocatalytic Cascades for Acylating N-Heterocycles with Natural Amino Acids via the 2-Keto Acids. Advanced Synthesis & Catalysis 2022, 364(3), 4013-4019. [link]

Zhu T^#, Cui YL^#, Geng WC, Wu B*, et al. Creating an Unusual Glycine-Rich Motif in a Peptide Amidase Leads to Versatile Protein C-Terminal Traceless Functionalization. ACS Catal. 2022, 12, 13, 8019-8026. [link]

Chen ML^#, Guo ZY^#, Sun JY^#, Tang W, Wu B*, Chen YH* Insights into the biosynthesis of septacidin l-heptosamine moiety unveils a VOC family sugar epimerase. Acta Pharm. Sin. B 2022, 2211-3835. [link]

Zhang ZL, Li PW, Wu B, Chen YH*, et al. (S)-3-aminopiperidine-2,6-dione is a biosynthetic intermediate of microbial blue pigment indigoidine. mLife 2022, 12023. [link]

Zheng Z, Ren SY, Wu B*, Wang H*, et al. Monitoring Methionine Decarboxylase by a Supramolecular Tandem Assay. Chem. Asian J. 2022, e202200106. [link]

Zhao X, Lian X, Wu B, Fu YV*, et al. A Peptide Derived from GAPDH Enhances Resistance to DNA Damage in Saccharomyces cerevisiae Cells. Appl Environ Microbiol. 2022, 88(4): e0219421. [link]

Sun JY, Wu B*, et al. Protein design with a machine-learned potential about backbone designability. Trends Biochem. Sci. 2022, 47(8), 638-640. [link]

Cui YL, Sun JY, Wu B*, et al. Computational enzyme redesign: large jumps in function. Trends in Chemistry 2022, 4(5): 409-419. [link]

2021

Ding Y, Li XL, Wu B, Chen YH*, et al. Construction of an Alternative NAD+ De Novo Biosynthesis Pathway. Adv. Sci. 2021, 8: 2004632. [link]

Chen ZH, Sun JY, Lou CB*, Wu B*, et al. Engineered DNase-inactive Cpf1 variants to improve targeting scope for base editing in E. coli. Synth. Syst. Biotechnol. 2021, 6(4): 326-334. [link]

Li RF^#, Schmidt M^#, Zhu T^#, Yang XY, Nuijens T*, Wu B*, et al. Traceless enzymatic protein synthesis without ligation sites constraint. Natl. Sci. Rev. 2021, nwab158. [link]

Feng J^#, Geng WC^#, Jiang HF, Wu B*. Recent advances in biocatalysis of nitrogen-containing heterocycles. Biotechnol. Adv. 2021, 107813. [link]

Cui YL^#, Wang YH^#, Tian WY^#, Wu B*, et al. Development of a versatile and efficient C-N lyase platform for asymmetric hydroamination via computational enzyme redesign. Nat. Catal. 2021, 4(5): 364-373. [link]

Cui YL^#, Chen YC^#, Liu XY, Wu B*, et al. Computational redesign of a PETase for plastic biodegradation under ambient condition by the GRAPE strategy. ACS Catal. 2021, 11(3): 1340-1350. [link]

Sun JY, Cui YL, Wu B*. GRAPE, a greedy accumulated strategy for computational protein engineering. Meth. Enzymol. 2021, 648: 207-230. [link]

杨新宇, 朱彤, 李瑞峰 吴边*. 从药物多肽到蛋白质全合成：酶促拼接的方法原理与前沿应用. 合成生物学 2021, 01: 33-45. [link]

孙瑨原, 崔颖璐 吴边*. 合成生物学中蛋白质计算预测设计的应用与发展. 生命科学 2021, 12: 1436-1444. [link]

孙瑨原, 朱彤, 李涛, 崔颖璐 吴边*. 微生物酶数据库的发展与应用. 微生物学报 2021, 12: 3783-3798. [link]

2020

Feng J^#, Li RF^#, Zhang SS^#, Bu YF, Wu B*, et al. Bioretrosynthesis of Functionalized N-Heterocycles from Glucose via One-Pot Tandem Collaborations of Designed Microbes. Adv. Sci. 2020, 202001188. [link]

Li T, Cui XX, Wu B*, et al. Exploration of Transaminase Diversity for the Oxidative Conversion of Natural Amino Acids into 2-Ketoacids and High-Value Chemicals. ACS. Catal. 2020, 10(14): 7950-7957. [link]

Zhu T^#, Li RF^#, Sun JY, Cui YL, Wu B*. Characterization and efficient production of a thermostable, halostable and organic solvent-stable cellulase from an oil reservoir. Int. J. Biol. Macromol. 2020, 159: 622-629. [link]

Li T^#, Li RF^#, Zhu T, Wu B*, et al. Improving the system performance of the asymmetric biosynthesis of D-pantoic acid by using artificially self-assembled enzymes in Escherichia coli. ACS. Biomater. Sci. Eng. 2020, 6(1): 219-224. [link]

Wang M, Xie ZJ, Wu B, Chen YH*, et al. Reductase of mutanobactin synthetase triggers sequential C-C macrocyclization, C-S bond formation, and C-C bond cleavage. Org. Lett. 2020, 22(3): 960-964. [link]

2019

Mu QX^#, Cui YL^#, Wu B*, et al. Thermostability improvement of the glucose oxidase from Aspergillus niger for efficient gluconic acid production via computational design. Int. J. Biol. Macromol. 2019, 136: 1060-1068. [link]

Zheng XX^#, Cui YL^#, Li T, Li RF, Guo L, Li DF, Wu B*. Biochemical and structural characterization of a highly active branched-chain amino acid aminotransferase from Pseudomonas sp. for efficient biosynthesis of chiral amino acid. Appl. Microbiol. Biotech. 2019, 103(19): 8051-8062. [link]

Li T^#, Cui YL^#, Wu B*. Molecular dynamics investigations of structural and functional changes in Bcl-2 induced by the novel antagonist BDA-366. J. Biomol. Struct. & Dyn. 2019, 37(10): 2527-2537. [link]

朱彤, 吴边*. 合成微生物组:当“合成生物学”遇见“微生物组学”. 科学通报 2019, (17), 1791-1798. [link]

曲戈, 朱彤, 蒋迎迎, 吴边, 孙周通.. 蛋白质工程：从定向进化到计算设计. 生物工程学报 2019, (10), 1843-1856. [link]

2018

Li RF^#, Wijma HJ^#, Song L^#, Cui YL, Otzen M, Tian YE, Du JW, Li T, Niu DD, Chen YC, Feng J, Han J, Chen H, Tao Y, Janssen DB*, Wu B*. Computational redesign of enzymes for regio- and enantioselective hydroamination. Nat. Chem. Biol. 2018, 14(7): 664-670. [link]

Bu YF^#, Cui YL^#, Peng Y, Hu MR, Tian Y’E, Tao Y, Wu B*. Engineering improved thermostability of the GH11 xylanase from Neocallimastix patriciarum via computational library design. Appl. Microbiol. Biotech. 2018, 102(8): 3675-3685. [link]

Zhu T, Song L, Li RF, Wu B*. Enzymatic clickable functionalization of peptides via computationally engineered peptide amidase. Chin. Chem. Lett. 2018, 29(7): 1116-1118. [link]

Tian XY^#, He GJ^#, Hu PJ^#, Chen L, Tao CY, Cui YL, Shen L, Ke WX, Xu HJ, Zhao YB, Xu QJ, Bai FY, Wu B, Yang E, Lin XR, Wang LQ*. Cryptococcus neoformans sexual reproduction is controlled by a quorum sensing peptide. Nat. Microbiol. 2018, 3(6): 698-707. [link]

崔颖璐, 吴边*. 符合工程化需求的生物元件设计. 中国科学院院刊 2018, (11): 1150-1157. [link]

2017

Feng J, Zhang P, Cui YL, Li K, Qiao X, Zhang YT, Li SM, Cox R.J, Wu B, Ye M*, Yin WB*. Regio- and stereospecific O-glycosylation of phenolic compounds catalyzed by a fungal glycosyltransferase from Mucor hiemalis. Adv. Synth. Catal. 2017, 359(6): 995-1006. [link]

崔颖璐, 吴边*. 计算机辅助蛋白结构预测及酶的计算设计研究进展. 广西科学 2017, (01), 1-6. [link]

2016

Wu B *, Wijma HJ, Song L, Rozeboom HJ, Poloni C, Tian YE, Arif MI, Nuijens T, Quaedflieg PJ, Szymanski W, Feringa BL, Janssen DB*. Versatile peptide C-terminal functionalization via a computationally engineered peptide amidase. ACS. Catal. 2016, 6(8): 5405-5414. [link]

Toplak A, Nuijens T, Quaedflieg PJ, Wu B*, Janssen DB*. Peptiligase, an enzyme for efficient chemoenzymatic peptide synthesis and cyclization in water. Adv. Synth. Catal. 2016, 358(13): 2140-2147. [link]

Nuijens T , Toplak A , Quaedflieg PJLM , Drenth J , Wu B*, Janssen DB*. Engineering a diverse ligase toolbox for peptide segment condensation. Adv. Synth. Catal. 2016, 358(24): 4041-4048. [link]