Publications

2023

Ren SY^#, Geng WC^#, Cui XX, Wu B*, Zheng Z*. A Label-Free and Continuous Fluorescence Detection for L-DOPA Decarboxylase Activity Based on Supramolecular Tandem Assay. ChemistrySelect 2023, 8(2), e202203646. [link]

Chen ML^#, Guo ZY^#, Sun JY^#, Tang W, Wang M, Tang Y, Li PW, Wu B*, Chen YH*. Insights into the biosynthesis of septacidin l-heptosamine moiety unveils a VOC family sugar epimerase. Acta Pharm. Sin. B 2023, 13(2), 765-774. [link]

2022

Cui XX^#, Geng WC^#, Jiang HF, Wu B*. Photobiocatalytic Cascades for Acylating N-Heterocycles with Natural Amino Acids via the 2-Keto Acids. Advanced Synthesis & Catalysis 2022, 364(3), 4013-4019. [link]

Zhu T^#, Cui YL^#, Geng WC, Liu GX, Jiang HF, Wu B*. Creating an Unusual Glycine-Rich Motif in a Peptide Amidase Leads to Versatile Protein C-Terminal Traceless Functionalization. ACS Catal. 2022, 12, 13, 8019-8026. [link]

Chen ML^#, Guo ZY^#, Sun JY^#, Tang W, Wang M, Tang Y, Li PW, Wu B*, Chen YH*. Insights into the biosynthesis of septacidin l-heptosamine moiety unveils a VOC family sugar epimerase. Acta Pharm. Sin. B 2022, 2211-3835. [link]

Zhang ZL, Li PW, Wang M, Zhang Yan, Wu B, Tao Y, Pan GH, Chen YH*. (S)-3-aminopiperidine-2,6-dione is a biosynthetic intermediate of microbial blue pigment indigoidine. mLife 2022, 12023. [link]

Zheng Z, Ren SY, Geng WC, Cui XX, Wu B*, Wang H*. Monitoring Methionine Decarboxylase by a Supramolecular Tandem Assay. Chem. Asian J. 2022, e202200106. [link]

Zhao X, Lian X, Liu Y, Zhou L, Wu B, Fu YV*. A Peptide Derived from GAPDH Enhances Resistance to DNA Damage in Saccharomyces cerevisiae Cells. Appl Environ Microbiol. 2022, 88(4): e0219421. [link]

Sun JY, Wu B*. Protein design with a machine-learned potential about backbone designability. Trends Biochem. Sci. 2022, 47(8), 638-640. [link]

Cui YL, Sun JY, Wu B*. Computational enzyme redesign: large jumps in function. Trends in Chemistry 2022, 4(5): 409-419. [link]

2021

Ding Y, Li XL, Horsman GP, Li PW, Wang M, Li J, Zhang ZL, Liu WF, Wu B, Tao Y, Chen YH*. Construction of an Alternative NAD+ De Novo Biosynthesis Pathway. Adv. Sci. 2021, 8: 2004632. [link]

Chen ZH, Sun JY, Guan Y, Li M, Lou CB*, Wu B*. Engineered DNase-inactive Cpf1 variants to improve targeting scope for base editing in E. coli. Synth. Syst. Biotechnol. 2021, 6(4): 326-334. [link]

Li RF^#, Schmidt M^#, Zhu T^#, Yang XY, Feng J, Tian YE, Cui YL, Nuijens T*, Wu B*. Traceless enzymatic protein synthesis without ligation sites constraint. Natl. Sci. Rev. 2021, nwab158. [link]

Feng J^#, Geng WC^#, Jiang HF, Wu B*. Recent advances in biocatalysis of nitrogen-containing heterocycles. Biotechnol. Adv. 2021, 107813. [link]

Cui YL^#, Wang YH^#, Tian WY^#, Bu YF, Li T, Cui XX, Zhu T, Li RF, Wu B*. Development of a versatile and efficient C-N lyase platform for asymmetric hydroamination via computational enzyme redesign. Nat. Catal. 2021, 4(5): 364-373. [link]

Cui YL^#, Chen YC^#, Liu XY, Dong SJ, Tian YE, Qiao YX, Mitra R, Han J, Li CL, Han X, Liu WD, Chen Q, Wei WQ, Wang X, Du, Tang SY, Xiang H, Liu HY, Liang Y, Houk KN, Wu B*. Computational redesign of a PETase for plastic biodegradation under ambient condition by the GRAPE strategy. ACS Catal. 2021, 11(3): 1340-1350. [link]

Sun JY, Cui YL, Wu B*. GRAPE, a greedy accumulated strategy for computational protein engineering. Meth. Enzymol. 2021, 648: 207-230. [link]

杨新宇, 朱彤, 李瑞峰 吴边*. 从药物多肽到蛋白质全合成：酶促拼接的方法原理与前沿应用. 合成生物学 2021, 01: 33-45. [link]

孙瑨原, 崔颖璐 吴边*. 合成生物学中蛋白质计算预测设计的应用与发展. 生命科学 2021, 12: 1436-1444. [link]

孙瑨原, 朱彤, 李涛, 崔颖璐 吴边*. 微生物酶数据库的发展与应用. 微生物学报 2021, 12: 3783-3798. [link]

2020

Feng J^#, Li RF^#, Zhang SS^#, Bu YF, Chen YC, Cui YL, Lin BX, Chen YH, Tao Y, Wu B*. Bioretrosynthesis of Functionalized N-Heterocycles from Glucose via One-Pot Tandem Collaborations of Designed Microbes. Adv. Sci. 2020, 202001188. [link]

Li T, Cui XX, Cui YL, Sun JY, Chen YC, Zhu T, Li CJ, Li RF, Wu B*. Exploration of Transaminase Diversity for the Oxidative Conversion of Natural Amino Acids into 2-Ketoacids and High-Value Chemicals. ACS. Catal. 2020, 10(14): 7950-7957. [link]

Zhu T^#, Li RF^#, Sun JY, Cui YL, Wu B*. Characterization and efficient production of a thermostable, halostable and organic solvent-stable cellulase from an oil reservoir. Int. J. Biol. Macromol. 2020, 159: 622-629. [link]

Li T^#, Li RF^#, Zhu T, Cui XX, Li CJ, Cui YL, Wu B*. Improving the system performance of the asymmetric biosynthesis of D-pantoic acid by using artificially self-assembled enzymes in Escherichia coli. ACS. Biomater. Sci. Eng. 2020, 6(1): 219-224. [link]

Wang M, Xie ZJ, Tang SB, Chang EL, Tang Y, Guo ZG, Cui YL, Wu B, Ye T, Chen YH*. Reductase of mutanobactin synthetase triggers sequential C-C macrocyclization, C-S bond formation, and C-C bond cleavage. Org. Lett. 2020, 22(3): 960-964. [link]

2019

Mu QX^#, Cui YL^#, Tian YE, Hu MR, Tao Y, Wu B*. Thermostability improvement of the glucose oxidase from Aspergillus niger for efficient gluconic acid production via computational design. Int. J. Biol. Macromol. 2019, 136: 1060-1068. [link]

Zheng XX^#, Cui YL^#, Li T, Li RF, Guo L, Li DF, Wu B*. Biochemical and structural characterization of a highly active branched-chain amino acid aminotransferase from Pseudomonas sp. for efficient biosynthesis of chiral amino acid. Appl. Microbiol. Biotech. 2019, 103(19): 8051-8062. [link]

Li T^#, Cui YL^#, Wu B*. Molecular dynamics investigations of structural and functional changes in Bcl-2 induced by the novel antagonist BDA-366. J. Biomol. Struct. & Dyn. 2019, 37(10): 2527-2537. [link]

朱彤, 吴边*. 合成微生物组:当“合成生物学”遇见“微生物组学”. 科学通报 2019, (17), 1791-1798. [link]

曲戈, 朱彤, 蒋迎迎, 吴边, 孙周通.. 蛋白质工程：从定向进化到计算设计. 生物工程学报 2019, (10), 1843-1856. [link]

2018

Li RF^#, Wijma HJ^#, Song L^#, Cui YL, Otzen M, Tian YE, Du JW, Li T, Niu DD, Chen YC, Feng J, Han J, Chen H, Tao Y, Janssen DB*, Wu B*. Computational redesign of enzymes for regio- and enantioselective hydroamination. Nat. Chem. Biol. 2018, 14(7): 664-670. [link]

Bu YF^#, Cui YL^#, Peng Y, Hu MR, Tian Y’E, Tao Y, Wu B*. Engineering improved thermostability of the GH11 xylanase from Neocallimastix patriciarum via computational library design. Appl. Microbiol. Biotech. 2018, 102(8): 3675-3685. [link]

Zhu T, Song L, Li RF, Wu B*. Enzymatic clickable functionalization of peptides via computationally engineered peptide amidase. Chin. Chem. Lett. 2018, 29(7): 1116-1118. [link]

Tian XY^#, He GJ^#, Hu PJ^#, Chen L, Tao CY, Cui YL, Shen L, Ke WX, Xu HJ, Zhao YB, Xu QJ, Bai FY, Wu B, Yang E, Lin XR, Wang LQ*. Cryptococcus neoformans sexual reproduction is controlled by a quorum sensing peptide. Nat. Microbiol. 2018, 3(6): 698-707. [link]

崔颖璐, 吴边*. 符合工程化需求的生物元件设计. 中国科学院院刊 2018, (11): 1150-1157. [link]

2017

Feng J, Zhang P, Cui YL, Li K, Qiao X, Zhang YT, Li SM, Cox R.J, Wu B, Ye M*, Yin WB*. Regio- and stereospecific O-glycosylation of phenolic compounds catalyzed by a fungal glycosyltransferase from Mucor hiemalis. Adv. Synth. Catal. 2017, 359(6): 995-1006. [link]

崔颖璐, 吴边*. 计算机辅助蛋白结构预测及酶的计算设计研究进展. 广西科学 2017, (01), 1-6. [link]

2016

Wu B *, Wijma HJ, Song L, Rozeboom HJ, Poloni C, Tian YE, Arif MI, Nuijens T, Quaedflieg PJ, Szymanski W, Feringa BL, Janssen DB*. Versatile peptide C-terminal functionalization via a computationally engineered peptide amidase. ACS. Catal. 2016, 6(8): 5405-5414. [link]

Toplak A, Nuijens T, Quaedflieg PJ, Wu B*, Janssen DB*. Peptiligase, an enzyme for efficient chemoenzymatic peptide synthesis and cyclization in water. Adv. Synth. Catal. 2016, 358(13): 2140-2147. [link]

Nuijens T , Toplak A , Quaedflieg PJLM , Drenth J , Wu B*, Janssen DB*. Engineering a diverse ligase toolbox for peptide segment condensation. Adv. Synth. Catal. 2016, 358(24): 4041-4048. [link]